The aim of this in vivo study was to evaluate the effect of two antimicrobial mouth rinses on dental plaque acidogenicity after a sucrose challenge.1

This double-blind, intra-individual randomised study assessed the effect of LISTERINE®, chlorhexidine (0.12%, alcohol-free) and water (as a negative control) on dental plaque acidogencity after a sucrose challenge (n=20).1.

The three mouth rinses were used in 16-day rinsing periods in addition to the subjects’ regular mechanical oral hygiene. The three test periods were separated by 3-month washout periods. Changes in plaque acidogenicity were evaluated after a sucrose challenge at day 0 (baseline) and at day 17 of each mouth rinse period using the microtouch method.1

Both LISTERINE® and the chlorhexidine rinse resulted in a statistically significant reduction in pH fall compared to the water rinse at day 17. There was no statistically significant difference between the two mouth rinses during the whole 30-minute period. When calculated as area under the curve for a pH of less than 6.2 (AUC6.2), significantly lower values were found for LISTERINE® and the chlorhexidine rinse at day 17 compared to day 0. Large inter-individual variations were observed.1

The results from this study indicate that both LISTERINE® and the alcohol-free chlorhexidine rinse reduced plaque acidogenicity after a sucrose challenge.1


  1. Albertsson K et al. Effects of mouthrinses containing essential oils and alcohol-free chlorhexidine on human plaque acidogenicity. Clin Oral Investig 2010; 14(1): 107–112.