Representative images of biofilm after 48 hours.

Untreated (negative control)

From left to right: Sagittal extended focus top down (growth surface on left), birds' eye view extended focus, 3D top view, 3D bottom view. Live bacteria are shown in green and dead bacteria in red.

LISTERINE® Coolmint treated biofilm

The visual results highlight the depth of penetration by LISTERINE® Coolmint showing more dead organisms throughout the biofilm after treatment

LISTERINE mouthrinse on biofilm growth in vitro

BioFilm NEW

SEM showing that LISTERINE® kills bacteria by penetrating and destroying the cell wall



SEM courtesy of Christoph Schaudinn, USC Centre for Biofilm Research, School of Dentistry.



  1. Fine DH et al. Comparative antimicrobial activities of antiseptic mouthrinses against isogenic planktonic and biofilm forms of Actinobacillus actinomycetemcomitans. J Clin Periodontol 2001; 28: 697–700.